Protein purification facility
About Us
The facility was founded in 2009 and aims to provide an extensive range of services to support those using and manipulating proteins in their research. Our goal is to be as quick, efficient and as helpful as possible. All work and discussions will be strictly private and confidential. Please read our Service level agreement [PDF 80KB].
Our Services
Our aim is to facilitate all stages of the purification process, from cloning design to the analysis of protein purity.
If you don't have the time to carry out all or some of the stages yourself, we have the expertise and equipment to do it for you. To use any of the equipment or take advantage of the complete purification service please get in touch for a consultation. Currently, the facility is only able to offer recombinant protein production in E. coli though there are plans to expand the facility to incorporate protein production in insect and mammalian cells.
Please fill in an ordering form [PDF 80 KB].
Equipment
Fermentor
Fermentors allow a high mass of bacterial cells, and hence the protein of interest, to be grown in a relatively small volume. We have a 5 litre fermentor from New Brunswick, which has constant monitoring of conditions such as pH, oxygen saturation and temperature. This allows cells to grow to very high density, typically to an OD 600 of greater than 20, in contrast to shaking flasks where the OD 600 generally reaches 2.
FPLC
Proteins destined for crystallisation must be homogenous and therefore require high resolution purification. Our ÄKTA FPLC is maintained at 4 C allows reliable, high performance protein purification. The system is automated yet versatile, with a user friendly software interface. The innovative compact design is optimised for accessibility and remote control of the system.
Charging
There are a range of charging options for the SBCS [PDF 68 KB] and external [PDF 64 KB] users. You can be charged for a breakdown of the experiments that you carry out and the equipment that you use. Alternatively, you can obtain a quote for a complete job. Contact us and the appropriate method can be identified to suit you best.