IUBMB Enzyme Nomenclature

EC 6.3.3.6

Accepted name: carbapenam-3-carboxylate synthase

Reaction: ATP + (2S,5S)-5-carboxymethylproline = AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate

Other name(s): CarA (ambiguous); CPS (ambiguous); carbapenam-3-carboxylate ligase

Systematic name: (2S,5S)-5-carboxymethylproline cyclo-ligase (AMP-forming)

Comments: The enzyme is involved in the biosynthesis of the carbapenem β-lactam antibiotic (5R)-carbapen-2-em-3-carboxylate in the bacterium Pectobacterium carotovorum.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number:

References:

1. Gerratana, B., Stapon, A. and Townsend, C.A. Inhibition and alternate substrate studies on the mechanism of carbapenam synthetase from Erwinia carotovora. Biochemistry 42 (2003) 7836-7847. [PMID: 12820893]

2. Miller, M.T., Gerratana, B., Stapon, A., Townsend, C.A. and Rosenzweig, A.C. Crystal structure of carbapenam synthetase (CarA). J. Biol. Chem. 278 (2003) 40996-41002. [PMID: 12890666]

3. Raber, M.L., Arnett, S.O. and Townsend, C.A. A conserved tyrosyl-glutamyl catalytic dyad in evolutionarily linked enzymes: carbapenam synthetase and β-lactam synthetase. Biochemistry 48 (2009) 4959-4971. [PMID: 19371088]

4. Arnett, S.O., Gerratana, B. and Townsend, C.A. Rate-limiting steps and role of active site Lys443 in the mechanism of carbapenam synthetase. Biochemistry 46 (2007) 9337-9345. [PMID: 17658887]

[EC 6.3.3.6 created 2013 as EC 6.3.1.16, transferred 2013 to EC 6.3.3.6]


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