Reaction: 4-chlorobenzoate + H2O = 4-hydroxybenzoate + chloride
Other name(s): halobenzoate dehalogenase
Systematic name: 4-chlorobenzoate chlorohydrolase
Comments: Catalyses the first step in the degradation of chlorobenzoate in Pseudomonas. In many microorganisms, this activity comprises three separate enzymes, EC 6.2.1.33 (4-chlorobenzoate—CoA ligase), EC 3.8.1.7 (4-chlorobenzoyl-CoA dehalogenase) and EC 3.1.2.23 (4-hydroxybenzoyl-CoA thioesterase).
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 94047-11-7
References:
1. Müller, R., Thiele, J., Klages, U. and Lingens, F. Incorporation of [18O]water into 4-hydroxybenzoic acid in the reaction of 4-chlorobenzoate dehalogenase from Pseudomonas spec. CBS 3. Biochem. Biophys. Res. Commun. 124 (1984) 178-182. [PMID: 6497878]
2. Heppel, L.A., Porterfield, V.T. Enzymatic dehalogenation of certain brominated and chlorinated compounds. J. Biol. Chem. 176 (1948) 763-769.