Reaction: 2,4-diacetylphloroglucinol + H2O = 2-acetylphloroglucinol + acetate
Glossary: phloroglucinol = benzene-1,3,5-triol
2,4-diacetylphloroglucinol = 1,1'-(2,4,6-trihydroxybenzene-1,3-diyl)diethan-1-one
Other name(s): PhlG
Systematic name: 2,4-diacetylphloroglucinol acetylhydrolase
Comments: Requires Zn2+. Isolated from the bacteria Pseudomonas fluorescens, Pseudomonas sp. YGJ3 and Mycobacterium abscessus 103. It reduces the antibiotic activity of 2,4-diacetylphloroglucinol.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number:
References:
1. Bottiglieri, M. and Keel, C. Characterization of PhlG, a hydrolase that specifically degrades the antifungal compound 2,4-diacetylphloroglucinol in the biocontrol agent Pseudomonas fluorescens CHA0. Appl. Environ. Microbiol. 72 (2006) 418-427. [PMID: 16391073]
2. He, Y.X., Huang, L., Xue, Y., Fei, X., Teng, Y.B., Rubin-Pitel, S.B., Zhao, H. and Zhou, C.Z. Crystal structure and computational analyses provide insights into the catalytic mechanism of 2,4-diacetylphloroglucinol hydrolase PhlG from Pseudomonas fluorescens. J. Biol. Chem. 285 (2010) 4603-4611. [PMID: 20018877]
3. Saitou, H., Watanabe, M. and Maruyama, K. Molecular and catalytic properties of 2,4-diacetylphloroglucinol hydrolase (PhlG) from Pseudomonas sp. YGJ3. Biosci. Biotechnol. Biochem. 76 (2012) 1239-1241. [PMID: 22790955]
4. Zhang, Z., Jiang, Y.L., Wu, Y. and He, Y.X. Crystallization and preliminary X-ray diffraction analysis of a putative carbon-carbon bond hydrolase from Mycobacterium abscessus 103. Acta Crystallogr. F Struct. Biol. Commun. 71 (2015) 239-242. [PMID: 25664803]