Reaction: α-D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3
For diagram click here.
Glossary: α-D-glucosamine 6-phosphate = 2-amino-2-deoxy-α-D-glucopyranose 6-phosphate
Other name(s): glucosaminephosphate isomerase (ambiguous); glucosamine-6-phosphate isomerase (ambiguous); phosphoglucosaminisomerase (ambiguous); glucosamine phosphate deaminase; aminodeoxyglucosephosphate isomerase (ambiguous); phosphoglucosamine isomerase (ambiguous); 2-amino-2-deoxy-D-glucose-6-phosphate aminohydrolase (ketol isomerizing)
Systematic name: 2-amino-2-deoxy-α-D-glucose-6-phosphate aminohydrolase (ketol isomerizing)
Comments: The enzyme uses ring opening and isomerization of the aldose-ketose type to convert the -CH(-NH2)-CH=O group of glucosamine 6-phosphate into -C(=NH)-CH2-OH, forming 2-deoxy-2-imino-D-arabino-hexitol, which then hydrolyses to yield fructose 6-phosphate and ammonia. N-Acetyl-D-glucosamine 6-phosphate, which is not broken down, activates the enzyme.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9013-10-9
References:
1. Wolfe, J.B., Britton, B.B., Nakada, H.I. Glucosamine degradation by Escherichia coli. III. Isolation and studies of "phosphoglucosaminisomerase". Arch. Biochem. Biophys. 66 (1957) 333-339. [PMID: 13403679]
2. Comb, D.G., Roseman, S. Glucosamine metabolism. IV. Glucosamine-6-phosphate deaminase. J. Biol. Chem. 232 (1958) 807-827. [PMID: 13549465]
3. Pattabiraman, T.N., Bachhawat, B.K. Purification of glucosamine 6-phosphate deaminase from human brain. Biochim. Biophys. Acta 54 (1961) 273-283. [PMID: 14484386]
4. Liu, C., Li, D., Liang, Y.H., Li, L.F. and Su, X.D. Ring-opening mechanism revealed by crystal structures of NagB and its ES intermediate complex. J. Mol. Biol. 379 (2008) 73-81. [PMID: 18436239]