IUBMB Enzyme Nomenclature


Accepted name: γ-glutamyl hydrolase

Reaction: Hydrolysis of a γ-glutamyl bond

Other names: conjugase; folate conjugase; lysosomal γ-glutamyl carboxypeptidase; γ-Glu-X carboxypeptidase; pteroyl-poly-γ-glutamate hydrolase; carboxypeptidase G; folic acid conjugase; poly(γ-glutamic acid) endohydrolase; polyglutamate hydrolase; poly(glutamic acid) hydrolase II; pteroylpoly-γ-glutamyl hydrolase

Comments: A lysosomal or secreted, thiol-dependent peptidase, most active at acidic pH. Commonly studied with folylpoly-γ-glutamate as substrate, with which the initial cleavage may release glutamate or poly-γ-glutamate of two or more residues, according to the species of origin of the enzyme. Final products are pteroyl-α-glutamate (folic acid) and free glutamate. Highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid (leaving group). Action on γ-glutamyl bonds is independent of an N-terminal pteroyl moiety, but it is not known whether an N-terminal γ-Glu residue can be hydrolysed. Type example of peptidase family C26. Formerly EC

Links to other databases: BRENDA, EXPASY, KEGG, GTD, MEROPS, Metacyc, PDB, CAS registry number: 9074-87-7


1. McGuire, J.J. and Coward, J.K. Pteroylpolyglutamates: biosynthesis, degradation and function. In: Folates and Pterins (Blakley, R.L. and Benkovic, S.J., eds.) pp. 135-191 (1984) John Wiley and Sons, New York

2. Wang, Y., Nimec, Z., Ryan, T.J., Dias, J.A. and Galivan, J. The properties of the secreted gamma-glutamyl hydrolases from H35 hepatoma cells. Biochim. Biophys. Acta 1164 (1993) 227-235 [PMID: 8343522]

3. Yao, R., Rhee, M.S. and Galivan, J. Effects of gamma-glutamyl hydrolase on folyl and antifolylpolyglutamates in cultured H35 hepatoma cells. Mol. Pharmacol. 48 (1995) 505-511 [PMID: 7565632]

4. Yao, R., Schneider, E., Ryan, T.J. and Galivan, J. Human gamma-glutamyl hydrolase: Cloning and characterization of the enzyme expressed in vitro. Proc. Natl. Acad. Sci. U. S. A. 93 (1996) 10134-10138 [PMID: 8816764]

5. Yao, R., Nimec, Z., Ryan, T.J. and Galivan, J. Identification, cloning, and sequencing of a cDNA coding for rat gamma-glutamyl hydrolase. J. Biol. Chem. 271 (1996) 8525-8528 [PMID: 8621474]

[EC created 1972 as EC, transferred 1978 to EC, transferred 1992 to EC, modified 1997]

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