Reaction: Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides
Other name(s): Escherichia coli exonuclease V; E. coli exonuclease V; gene recBC endoenzyme; RecBC deoxyribonuclease; gene recBC DNase; exonuclease V; gene recBCD enzymes
Comments: Preference for double-stranded DNA. Possesses DNA-dependent ATPase activity. Acts endonucleolytically on single-stranded circular DNA.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 37350-26-8
References:
1. Eichler, D.C. and Lehman, I.R. On the role of ATP in phosphodiester bond hydrolysis catalyzed by the recBC deoxyribonuclease of Escherichia coli. J. Biol. Chem. 252 (1977) 499-503. [PMID: 319095]
2. Goldmark, P.J. and Liun, S. Purification and properties of the recBC DNase of Escherichia coli K-12. J. Biol. Chem. 247 (1972) 1849-1860. [PMID: 4552016]
3. Oishi, M. An ATP-dependent deoxyribonuclease from Escherichia coli with a possible role in genetic recombination. Proc. Natl. Acad. Sci. USA 64 (1969) 1292-1299. [PMID: 4916924]
4. Wright, M., Buttin, G. and Hurwitz, J. The isolation and characterization from Escherichia coli of an adenosine triphosphate-dependent deoxyribonuclease directed by rec B, C genes. J. Biol. Chem. 246 (1971) 6543-6555. [PMID: 4332130]