Reaction: 3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
Glossary: 3'-phosphoadenylyl sulfate = PAPS
Other names: heparin N-sulfotransferase; 3'-phosphoadenylylsulfate:N-desulfoheparin sulfotransferase; PAPS:N-desulfoheparin sulfotransferase; PAPS:DSH sulfotransferase; N-HSST; N-heparan sulfate sulfotransferase; heparan sulfate N-deacetylase/N-sulfotransferase; heparan sulfate 2-N-sulfotransferase; heparan sulfate N-sulfotransferase; heparan sulfate sulfotransferase; N-desulfoheparin sulfotransferase; desulfoheparin sulfotransferase; 3'-phosphoadenylyl-sulfate:N-desulfoheparin N-sulfotransferase; heparitin sulfotransferase; 3'-phosphoadenylyl-sulfate:heparitin N-sulfotransferase; 3'-phosphoadenylyl-sulfate:[heparan sulfate]-glucosamine N-sulfotransferase
Systematic name: 3'-phosphoadenylyl-sulfate:[heparan sulfate]-glucosamine N-sulfonotransferase
Comments: The enzyme also catalyses the sulfation of chondroitin 4-sulfate and dermatan sulfate, but to a much more limited extent.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9026-75-9
References:
1. Suzuki, S., Trenn, R.H. and Strominger, J.L. Separation of specific mucopolysaccharide sulfotransferases. Biochim. Biophys. Acta 50 (1961) 169-174.
2. Eisenman, R.A., Balasubramanian, A.S. and Marx, W. 3'-Phosphoadenylylsulfate:N-desulfoheparin sulfotransferase associated with a postmicrosomal particulate mastocytoma fraction. Arch. Biochem. Biophys. 119 (1967) 387-397. [PMID: 4964017]
3. Johnson, A.H. and Baker, J.R. The enzymatic sulphation of heparan sulphate by hen's uterus. Biochim. Biophys. Acta 320 (1973) 341-351. [PMID: 4270798]