IUBMB Enzyme Nomenclature

EC 2.8.2.32

Accepted name: scymnol sulfotransferase

Reaction: 3'-phosphoadenylyl sulfate + 5β-scymnol = adenosine 3',5'-bisphosphate + 5β-scymnol sulfate

For diagram click here.

Glossary: 3'-phosphoadenylyl sulfate = PAPS
5β-scymnol sulfate = (24R,25S)-3α,7α,12α,24,27-pentahydroxy-5β-cholestan-26-yl sulfate
5α-cyprinol = 5α-cholestane-3α,7α,12α,26,27-pentol

Other names: 3'-phosphoadenosine 5'-phosphosulfate:5β-scymnol sulfotransferase; 3′-phosphoadenylyl sulfate:5β-scymnol sulfotransferase

Systematic name: 3′-phosphoadenylyl sulfate:5β-scymnol sulfonotransferase

Comments: The enzyme from the shark Heterodontus portusjacksoni is able to sulfate the C27 bile salts 5β-scymnol (the natural bile salt) and 5α-cyprinol (the carp bile salt). Enzyme activity is activated by Mg2+ but inhibited by the product 5β-scymnol sulfate.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 220581-70-4

References:

1. Macrides, T.A., Faktor, D.A., Kalafatis, N. and Amiet, R.G. Enzymic sulfation of bile salts. Partial purification and characterization of an enzyme from the liver of the shark Heterodontus portusjacksoni that catalyses the sulfation of the shark bile steroid 5β-scymnol. Comp. Biochem. Physiol. Part B 107 (1994) 461-469. [PMID: 7749614]

2. Pettigrew, N.E., Wright, P.F.A. and Macrides, T.A. Investigation of 5β-scymnol sulfotransferase from the kidney and testes of Heterodontus portusjacksoni. Comp. Biochem. Physiol. Part B 121 (1998) 243-249.

3. Pettigrew, N.E., Wright, P.F.A. and Macrides, T.A. 5β-Scymnol sulfotransferase isolated from the tissues of an Australian shark species. Comp. Biochem. Physiol. Part B 121 (1998) 299-307.

4. Pettigrew, N.E., Wright, P.F.A. and Macrides, T.A. 5β-scymnol sulfotransferase isolated from the liver of two Australian ray species. Comp. Biochem. Physiol. Part B 121 (1998) 341-348.

[EC 2.8.2.32 created 2005]


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