Reaction: nucleoside triphosphate + gentamicin = diphosphate + 2"-nucleotidylgentamicin
Other names: gentamicin 2"-adenylyltransferase; aminoglycoside adenylyltransferase; gentamycin 2"-nucleotidyltransferase
Systematic name: NTP:gentamicin 2"-nucleotidyltransferase
Comments: ATP, dATP, CTP, ITP and GTP can act as donors; kanamycin, tobramycin and sisomicin can also act as acceptors. The nucleotidyl residue is transferred to the 2-hydroxy of the 3-amino-3-deoxy-D-glucose moiety in the antibiotic.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 62213-33-6
References:
1. Angelatou, F., Litsas, S.B. and Kontomichalou, P. Purification and properties of two gentamicin-modifying enzymes, coded by a single plasmid pPK237 originating from Pseudomonas aeruginosa. J. Antibiot. 35 (1982) 235-244. [PMID: 6281224]
2. Naganawa, H., Yagisawa, M., Kondo, S., Takeuchi, T. and Umezawa, H. The structure determination of an enzymatic inactivation product of 3',4'-dideoxykanamycin B. J. Antibiot. 24 (1971) 913-914. [PMID: 4946513]
3. Yagisawa, M., Naganawa, H., Kondo, S., Hamada, M., Takeuchi, T. and Umezawa, H. Adenylyldideoxykanamycin B, a product of the inactivation of dideoxykanamycin B by Escherichia coli carrying R factor. J. Antibiot. 24 (1971) 911-912. [PMID: 4946512]