Reaction: ATP + kanamycin = ADP + kanamycin 3'-phosphate
Glossary: kanamycin
Other name(s): neomycin-kanamycin phosphotransferase;
kanamycin kinase (phosphorylating); neomycin phosphotransferase
Systematic name: ATP:kanamycin 3'-O-phosphotransferase
Comments: Also acts on the antibiotics neomycin, paromomycin, neamine, paromamine, vistamycin and gentamicin A. An enzyme from Pseudomonas aeruginosa also acts on butirosin.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 62213-36-9
References:
1. Doi, O., Ogura, M., Tanaka, N. and Umezawa, H. Inactivation of kanamycin, neomycin, and streptomycin by enzymes obtained in cells of Pseudomonas aeruginosa. Appl. Microbiol. 16 (1968) 1276-1281. [PMID: 4970990]
2. Dolin, M.I. The Streptococcus faecalis oxidases for reduced diphosphopyridine nucleotide. III. Isolation and properties of a flavin peroxidase for reduced diphosphopyridine nucleotide. J. Biol. Chem. 225 (1957) 557-573.