Reaction: phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O = phosphate + N-acetylneuraminate
For diagram of reaction click here and mechanism click here.
Other name(s): (NANA)condensing enzyme; N-acetylneuraminate pyruvate-lyase (pyruvate-phosphorylating); NeuAc synthase
Systematic name: phosphoenolpyruvate:N-acetyl-D-mannosamine C-(1-carboxyvinyl)transferase (phosphate-hydrolysing, 2-carboxy-2-oxoethyl-forming)
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 37290-66-7
1. Blacklow, R.S. and Warren, L. Biosynthesis of sialic acids by Neisseria meningitidis. J. Biol. Chem. 237 (1962) 3520-3526.
2. Komaki, E., Ohta, Y. and Tsukada, Y. Purification and characterization of N-acetylneuraminate synthase from Escherichia coli K1-M12. Biosci. Biotechnol. Biochem. 61 (1997) 2046-2050. [PMID: 9438985]