IUBMB Enzyme Nomenclature

EC 2.4.1.78

Accepted name: phosphopolyprenol glucosyltransferase

Reaction: UDP-glucose + undecaprenyl phosphate = UDP + β-D-glucosyl-1-phosphoundecaprenol

Other name(s): gtrB (gene name); bactoprenol glucosyl transferase; polyisoprenyl-phosphate glycosyltransferase; uridine diphosphoglucose-polyprenol monophosphate glucosyltransferase; UDP-glucose:polyprenol monophosphate glucosyltransferase

Systematic name: UDP-glucose:undecaprenyl-phosphate D-glucosyltransferase (configuration- inverting)

Comments: The enzyme, characterized from the bacterium Shigella flexneri and its bacteriophages, catalyses the transfer of the glucose residue from UDP-glucose to the lipid carrier undecaprenyl phosphate. Following the flipping of the lipid-bound glucose across the cytoplasmic membrane, the glucose is used in the synthesis of the organism’s O-glycan in the periplasm.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 55576-46-0

References:

1. Jankowski, W., Mankowski, T. and Chojnacki, T. Formation of polyprenol monophosphate glucose in Shigella flexneri. Biochim. Biophys. Acta 337 (1974) 153-162. [PMID: 4373050]

2. Mavris, M., Manning, P.A. and Morona, R. Mechanism of bacteriophage SfII-mediated serotype conversion in Shigella flexneri. Mol. Microbiol. 26 (1997) 939-950. [PMID: 9426131]

3. Allison, G.E. and Verma, N.K. Serotype-converting bacteriophages and O-antigen modification in Shigella flexneri. Trends Microbiol 8 (2000) 17-23. [PMID: 10637639]

4. Ardiccioni, C., Clarke, O.B., Tomasek, D., Issa, H.A., von Alpen, D.C., Pond, H.L., Banerjee, S., Rajashankar, K.R., Liu, Q., Guan, Z., Li, C., Kloss, B., Bruni, R., Kloppmann, E., Rost, B., Manzini, M.C., Shapiro, L. and Mancia, F. Structure of the polyisoprenyl-phosphate glycosyltransferase GtrB and insights into the mechanism of catalysis. Nat Commun 7 (2016) 10175. [PMID: 26729507]

[EC 2.4.1.78 created 1976]


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