Reaction: (1) dolabradiene + O2 + [reduced NADPH—hemoprotein reductase] = 15,16-epoxydolabrene + H2O + [oxidized NADPH—hemoprotein reductase]
(2) 15,16-epoxydolabrene + O2 + [reduced NADPH—hemoprotein reductase] = 3β-hydroxy-15,16-epoxydolabrene + H2O + [oxidized NADPH—hemoprotein reductase]
For diagram of reaction click here.
Glossary: dolabradiene = (4aS,4bR,7S,8aR,10aS)-7-ethenyl-4b,7,10a-trimethyl-1-methylidenetetradecahydrophenanthrene
Other name(s): CYP71Z16 (gene name)
Systematic name: dolabradiene,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (3β-hydroxy-15,16-epoxydolabrene-forming)
Comments: A cytochrome P-450 (heme thiolate) enzyme characterized from maize. The enzyme catalyses the epoxidation of dolabradiene at C-16, followed by hydroxylation at C-3.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
References:
1. Mafu, S., Ding, Y., Murphy, K.M., Yaacoobi, O., Addison, J.B., Wang, Q., Shen, Z., Briggs, S.P., Bohlmann, J., Castro-Falcon, G., Hughes, C.C., Betsiashvili, M., Huffaker, A., Schmelz, E.A. and Zerbe, P. Discovery, biosynthesis and stress-related accumulation of dolabradiene-derived defenses in maize. Plant Physiol. 176 (2018) 2677-2690. [PMID: 29475898]