Reaction: lithocholate + NADPH + H+ + O2 = 6β-hydroxylithocholate + NADP+ + H2O
For diagram click here.
Glossary: lithocholic acid = 3α-hydroxy-5β-cholan-24-oic acid
murideoxycholic acid = 3α,6β-dihydroxy-5β-cholan-24-oic acid
Other name(s): lithocholate 6β-monooxygenase; CYP3A10; 6β-hydroxylase; cytochrome P450 3A10/lithocholic acid 6β-hydroxylase
Systematic name: lithocholate,NADPH:oxygen oxidoreductase (6β-hydroxylating)
Comments: A heme-thiolate protein (P-450). Expression of the gene for this enzyme is 50-fold higher in male compared to female hamsters .
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 9075-83-6
1. Teixeira, J. and Gil, G. Cloning, expression, and regulation of lithocholic acid 6β-hydroxylase. J. Biol. Chem. 266 (1991) 21030-21036. [PMID: 1840595]
2. Chang, T.K., Teixeira, J., Gil, G. and Waxman, D.J. The lithocholic acid 6β-hydroxylase cytochrome P-450, CYP 3A10, is an active catalyst of steroid-hormone 6β-hydroxylation. Biochem. J. 291 (1993) 429-433. [PMID: 8484723]
3. Subramanian, A., Wang, J. and Gil, G. STAT 5 and NF-Y are involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6β-hydroxylase. Nucleic Acids Res. 26 (1998) 2173-2178. [PMID: 9547277]
4. Russell, D.W. The enzymes, regulation, and genetics of bile acid synthesis. Annu. Rev. Biochem. 72 (2003) 137-174. [PMID: 12543708]