Reaction: an isoflavone + NADPH + H+ + O2 = a 2'-hydroxyisoflavone + NADP+ + H2O
For diagram click here.
Other name(s): isoflavone 2'-monooxygenase; CYP81E1; CYP Ge-3
Systematic name: isoflavone,NADPH:oxygen oxidoreductase (2'-hydroxylating)
Comments: A heme-thiolate protein (P-450). Acts on daidzein, formononetin and genistein. EC 18.104.22.168, 4'-methoxyisoflavone 2'-hydroxylase, has the same reaction but is more specific as it requires a 4'-methoxyisoflavone.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 110183-49-8
1. Akashi, T., Aoki, T. and Ayabe, S.-I. CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase. Biochem. Biophys. Res. Commun. 251 (1998) 67-70. [PMID: 9790908]