Reaction: anthranilate + NADPH + H+ + O2 = 2,3-dihydroxybenzoate + NADP+ + NH3
Other name(s): anthranilate hydroxylase; anthranilate 2,3-dioxygenase (deaminating); anthranilate hydroxylase (deaminating); anthranilic hydroxylase; anthranilate 2,3-hydroxylase (deaminating)
Systematic name: anthranilate,NADPH:oxygen oxidoreductase (3-hydroxylating, deaminating)
Comments: The enzyme from Aspergillus niger is an iron protein; that from the yeast Trichosporon cutaneum is a flavoprotein (FAD). Previously EC 18.104.22.168.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, UM-BBD, CAS registry number: 37256-68-1
1. Powlowski, J.B., Dagley, S., Massey, V. and Ballou, D.P. Properties of anthranilate hydroxylase (deaminating), a flavoprotein from Trichosporon cutaneum. J. Biol. Chem. 262 (1987) 69-74. [PMID: 3793735]
2. Subramanian, V. and Vaidyanathan, C.S. Anthranilate hydroxylase from Aspergillus niger: new type of NADPH-linked nonheme iron monooxygenase. J. Bacteriol. 160 (1984) 651-655. [PMID: 6501219]