IUBMB Enzyme Nomenclature

EC 1.14.13.162

Accepted name: (+)-camphor 6-exo-hydroxylase

Accepted name: 2,5-diketocamphane 1,2-monooxygenase

Reaction: (+)-bornane-2,5-dione + O2 + NADH + H+ = (+)-5-oxo-1,2-campholide + NAD+ + H2O

For diagram of reaction click here.

Glossary: 2,5-diketocamphane = (+)-bornane-2,5-dione

Other name(s): 2,5-diketocamphane lactonizing enzyme; ketolactonase I (ambiguous); 2,5-diketocamphane 1,2-monooxygenase oxygenating component; 2,5-DKCMO; camP (gene name); camphor 1,2-monooxygenase; camphor ketolactonase I

Systematic name: (+)-bornane-2,5-dione,NADH:oxygen oxidoreductase (1,2-lactonizing)

Comments: A flavoprotein (FMN) which requires Fe2+. A Baeyer-Villiger monooxygenase isolated from camphor-grown strains of Pseudomonas putida and encoded on the cam plasmid. Involved in the degradation of (+)-camphor. Requires a dedicated NADH-FMN reductase [cf. EC 1.5.1.42, FMN reductase (NADH)] [1-3]. Can accept several bicyclic ketones including (+)- and (–)-camphor [6] and adamantanone [4]. The product spontaneously converts to [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, UM-BBD, CAS registry number: 37256-81-8

References:

1. Conrad, H.E., DuBus, R., Namtvedt, M.J. and Gunsalus, I.C. Mixed function oxidation. II. Separation and properties of the enzymes catalyzing camphor lactonizaton. J. Biol. Chem. 240 (1965) 495-503. [PMID: 14253460]

2. Yu, C.A. and Gunsalus, I.C. Monoxygenases. VII. Camphor ketolactonase I and the role of three protein components. J. Biol. Chem. 244 (1969) 6149-6152. [PMID: 4310834]

3. Taylor, D.G. and Trudgill, P.W. Camphor revisited: studies of 2,5-diketocamphane 1,2-monooxygenase from Pseudomonas putida ATCC 17453. J. Bacteriol. 165 (1986) 489-497. [PMID: 3944058]

4. Selifonov, S.A. Microbial oxidation of adamantanone by Pseudomonas putida carrying the camphor catabolic plasmid. Biochem. Biophys. Res. Commun. 186 (1992) 1429-1436. [PMID: 1510672]

5. Jones, K.H., Smith, R.T. and Trudgill, P.W. Diketocamphane enantiomer-specific 'Baeyer-Villiger' monooxygenases from camphor-grown Pseudomonas putida ATCC 17453. J. Gen. Microbiol. 139 (1993) 797-805. [PMID: 8515237]

6. Kadow, M., Sass, S., Schmidt, M. and Bornscheuer, U.T. Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007. AMB Express 1 (2011) 13. [PMID: 21906366]

[EC 1.14.13.162 created 1972 as EC 1.14.15.2, transferred 2012 to EC 1.14.13.162]


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