IUBMB Enzyme Nomenclature

EC 1.14.13.158

Accepted name: amorpha-4,11-diene 12-monooxygenase

Reaction: amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+ = artemisinate + 3 NADP+ + 4 H2O (overall reaction)
(1a) amorpha-4,11-diene + O2 + NADPH + H+ = artemisinic alcohol + NADP+ + H2O
(1b) artemisinic alcohol + O2 + NADPH + H+ = artemisinic aldehyde + NADP+ + 2 H2O
(1c) artemisinic aldehyde + O2 + NADPH + H+ = artemisinate + NADP+ + H2O

For diagram of reaction click here.

Other name(s): CYP71AV1

Systematic name: amorpha-4,11-diene,NADPH:oxygen oxidoreductase (12-hydroxylating)

Comments: A heme-thiolate protein (P-450). Cloned from the plant Artemisia annua (sweet wormwood). Part of the biosynthetic pathway of artemisinin.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:

References:

1. Teoh, K.H., Polichuk, D.R., Reed, D.W., Nowak, G. and Covello, P.S. Artemisia annua L. (Asteraceae) trichome-specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin. FEBS Lett. 580 (2006) 1411-1416. [PMID: 16458889]

[EC 1.14.13.158 created 2012]


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