Reaction: geraniol + NADPH + H+ + O2 = (6E)-8-hydroxygeraniol + NADP+ + H2O
For diagram of reaction click here.
Other name(s): CYP76B6; G10H; CrG10H; SmG10H
Systematic name: geraniol,NADPH:oxygen oxidoreductase (8-hydroxylating)
Comments: Requires cytochrome P450. Also hydroxylates nerol and citronellol, cf. EC 188.8.131.52 linalool 8-monooxygenase. The recommended numbering of geraniol gives 8-hydroxygeraniol as the product rather than 10-hydroxygeraniol as used by references 1-3. See prenol nomenclature Pr-1. The cloned enzyme also catalysed, but less efficiently, the 3'-hydroxylation of naringenin (cf. EC 184.108.40.206, flavonoid 3'-monooxygenase) .
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
1. Collu, G., Unver, N., Peltenburg-Looman, A.M., van der Heijden, R., Verpoorte, R. and Memelink, J. Geraniol 10-hydroxylase, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis. FEBS Lett. 508 (2001) 215-220. [PMID: 11718718]
2. Wang, J., Liu, Y., Cai, Y., Zhang, F., Xia, G. and Xiang, F. Cloning and functional analysis of geraniol 10-hydroxylase, a cytochrome P450 from Swertia mussotii Franch. Biosci. Biotechnol. Biochem. 74 (2010) 1583-1590. [PMID: 20699579]
3. Sung, P.H., Huang, F.C., Do, Y.Y. and Huang, P.L. Functional expression of geraniol 10-hydroxylase reveals its dual function in the biosynthesis of terpenoid and phenylpropanoid. J. Agric. Food Chem. 59 (2011) 4637-4643. [PMID: 21504162]