Reaction: N1-methyladenine in mRNA + 2-oxoglutarate + O2 = adenine in mRNA + formaldehyde + succinate + CO2
Other name(s): ALKBH3
Systematic name: mRNA-N1-methyladenine,2-oxoglutarate:oxygen oxidoreductase (formaldehyde-forming)
Comments: Contains iron(II). Catalyses oxidative demethylation of mRNA N1-methyladenine. The enzyme is also involved in alkylation repair in DNA [2].
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number:
References:
1. Sundheim, O., Vågbø, C.B., Bjørås, M., Sousa, M.M., Talstad, V., Aas, P.A., Drabløs, F., Krokan, H.E., Tainer, J.A. and Slupphaug, G. Human ABH3 structure and key residues for oxidative demethylation to reverse DNA/RNA damage. EMBO J. 25 (2006) 3389-3397. [PMID: 16858410]
2. Dango, S., Mosammaparast, N., Sowa, M.E., Xiong, L.J., Wu, F., Park, K., Rubin, M., Gygi, S., Harper, J.W. and Shi, Y. DNA unwinding by ASCC3 helicase is coupled to ALKBH3-dependent DNA alkylation repair and cancer cell proliferation. Mol. Cell 44 (2011) 373-384. [PMID: 22055184]
3. Li, X., Xiong, X., Wang, K., Wang, L., Shu, X., Ma, S. and Yi, C. Transcriptome-wide mapping reveals reversible and dynamic N-methyladenosine methylome. Nat. Chem. Biol. (2016) . [PMID: 26863410]