Reaction: testosterone + NAD(P)+ = androstenedione + NAD(P)H + H+
Glossary: androstenedione = androst-4-ene-3,17-dione
Other name(s): β-hydroxy steroid dehydrogenase; 17-ketoreductase; 17β-hydroxy steroid dehydrogenase; 3β-hydroxysteroid dehydrogenase; 17β-hydroxy steroid dehydrogenase; 3β-hydroxy steroid dehydrogenase
Systematic name: 3(or 17)β-hydroxysteroid:NAD(P)+ oxidoreductase
Comments: Also acts on other 3β- or 17β-hydroxysteroids. cf. EC 1.1.1.209 3(or 17)α-hydroxysteroid dehydrogenase.
Links to other databases: BRENDA, EAWAG-BBD, EXPASY, GTD, KEGG, Metacyc, PDB, CAS registry number: 9015-81-0
References:
1. Dahm, K. and Breuer, H. Anreicherung einer 17β-hydroxysteroid:NAD(P)-oxydoreduktase aus der Nebenniere der Ratte. Hoppe-Seyler's Z. Physiol. Chem. 336 (1964) 63-68.
2. Lynn, W.S. and Brown, R.H. The conversion of progesterone to androgens by testes. J. Biol. Chem. 232 (1958) 1015-1030.
3. Marcus, P.I. and Talalay, P. Induction and purification of α- and β-hydroxysteroid dehydrogenases. J. Biol. Chem. 218 (1956) 661-674.
4. Schultz, R.M., Groman, F.V. and Engel, L.L. 3(17)β-Hydroxysteroid dehydrogenase of Pseudomonas testosteroni. A convenient purification and demonstration of multiple molecular forms. J. Biol. Chem. 252 (1977) 3775-3783. [PMID: 193845]
5. Talalay, P. and Dobson, M.M. Purification and properties of a α-hydroxysteroid dehydrogenase. J. Biol. Chem. 205 (1953) 823-837.